Aromatization of 19-norandrogens by equine testicular microsomes.

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Aromatization of 19-norandrogens by equine testicular microsomes.

In the stallion testis, aromatase activity was localized in the microsomal fraction. Androgen aromatization occurred through the loss of 1 beta,2 beta hydrogen atoms and appeared to involve free sulfhydryl groups. A single enzyme system seemed to aromatize androgen and norandrogen at the same rate while having a much lower affinity for norandrogens.

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Androgen synthesis and aromatization by equine corpus luteum microsomes.

Whereas mare corpus luteum does not produce androgens or estrogens in vivo, the incubation of mare corpus luteum microsomes with progesterone and NADPH resulted in 17 alpha-hydroxyprogesterone and estrogen production with a small yield of androstenedione. In the presence of an aromatase inhibitor (4-hydroxyandrostenedione), 17 alpha-hydroxyprogesterone and androstenedione were accumulated. Arom...

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Lipid peroxidation in adrenal and testicular microsomes.

Studies were carried out to determine the actions of and interactions between ascorbate, NADPH, Fe2+, and Fe3+ on lipid peroxidation in adrenal and testicular microsomes. Ascorbate-induced malonaldehyde production was maximal in adrenal and testicular microsomes at an ascorbate concentration of 1 X 10(-4)M. Fe2+, at levels between 10(-6) and 10(-3)M, produced concentration-dependent increases i...

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Testicular vasculitis: a series of 19 cases.

OBJECTIVES Because of limited reported cases, it is unknown how often testicular vasculitis represents isolated or systemic disease. METHODS We report 19 cases (15 consultation; 4 in-house cases) of localized testicular infarction with associated vasculitis spanning 24 years. RESULTS All were orchiectomy specimens; detailed clinical information was available in 16 cases. Mean age was 38 yea...

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Utilization of oxygen and reduced nicotinamide adenine dinucleotide phosphate by human placental microsomes during aromatization of androstenedione.

The addition of androstenedione and NADPH to human placental microsomes results in a marked increase in the rate of 0 2 and NADPH utilization concomitant with estrogen formation. Initially, 5 to 12 moles of O2 and an equivalent amount of NADPH are consumed per mole of estrogen formed, the ratio varying from one microsomal preparation to another. In the presence, however, of 10 to 20 mM KCN, 3 m...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1987

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)45634-7